Smart light-sheet fluorescence microscopy
The light-sheet fluorescence microscope is used to study three-dimensional samples combining the advantages of the speed of traditional wide-field epi-fluorescence microscopy with the optical sectioning power of a confocal microscope. This combination makes it an ideal choice for the study of three-dimensional samples with high temporal dynamics. The problem lies in that all biological samples are optical inhomogeneous leading to the introduction of phase aberrations that cause the images registered by these microscopes to be degraded and constitute a loss in information in the imaging process. Through adaptive optical elements this missing information can be found and the true distribution of fluorescence can be elucidated.
To find novel techniques that apply adaptive optics to light-sheet fluorescence microscopy in order to obtain better images and more information from biological samples.
We have to date produced methodologies for changing the shape of the incident light to be better suited for light-sheet imaging; we have corrected aberrations in the illumination path and found a technique to correct for aberrations in the detected fluorescence light.
- Dean Wilding
- Paolo Pozzi
- Oleg Soloviev
- Gleb Vdovin
- Michel Verhaegen
adaptive optics, microscopy, deconvolution
European Research Council (ERC) – iCON Project
University of Texas Southwestern
(1) Design and shaping of light for illumination.
(2) Correction of aberrations in illumination.
(3) Correction of aberrations in detection.
- Wilding, Dean, et al. "Pupil filters for extending the field-of-view in light-sheet microscopy." Optics letters (2016)
- Wilding, Dean, et al. "Adaptive illumination based on direct wavefront sensing in a light-sheet fluorescence microscope." Optics express (2016)
- Wilding, Dean, et al. "Blind multiframe deconvolution by iterative tangential projections." Optics express (2017)