Angelo Accardo

Assistant professor

Dr. Angelo Accardo is Assistant Professor of Soft Micro- and Nanosystem Technology for Life Sciences and Biology at Delft University of Technology (TU Delft), Faculty of Mechanical, Maritime and Materials Engineering (3mE), Department of Precision and Microsystems Engineering (PME) since June 1st 2019.

Dr. Angelo Accardo received his Master's degree in Electronic Engineering from the University of Rome “La Sapienza”, Italy (2008) and his Ph. D. in Biomedical Engineering from the University Magna Græcia of Catanzaro, Italy (2012) in collaboration with the European Synchrotron Radiation Facility (ESRF) in Grenoble, France. After a Post-doc at the Italian Institute of Technology (IIT) in Genova, Italy (2012-2015), Dr. Accardo joined the Laboratory for Analysis and Architecture of Systems (LAAS-CNRS), Toulouse, France in 2016 as a Research Associate. In June 2019, He joins Delft University of Technology (TU Delft) as a Tenure Track Assistant Professor of Soft Micro- and Nanosystem Technology for Life Sciences and Biology within the Department of Precision and Microsystems Engineering (PME). During his Ph. D. and Post-doctoral activity, Dr. Accardo acquired a strong knowledge of nano-fabrication processes in clean room facilities by means of which he designed several technological platforms that have been integrated for the first time in the context of synchrotron radiation scattering characterization of biological soft matter compounds (e.g. neurodegenerative peptides, cancer cell exosomes). The main topic under investigation was the realization of superhydrophobic surfaces made of different materials, such as polymer and silicon, following a biomimetic approach and exploiting the characteristic evaporation mechanisms of droplets drying in quasi contact-free conditions for tuning self-assembly and crystallization mechanisms. Since a few years, his main area of research focuses instead on the development of multi-scale (nano-micro-meso) 3D fabrication paradigms to address a wide range of open questions in cell biology. He is interested in particular in the design and fabrication of 3D microenvironments, by employing light-assisted additive manufacturing techniques (such as two-photon lithography and stereolithography), for studying the mechanobiology, migration and differentiation mechanisms of neuronal cells as well as of neural spheroids/organoids. He also employs multi-technique 3D imaging protocols combining conventional morphological characterization techniques (based on Scanning Electron Microscopy) and advanced 3D fluorescence imaging (Light Sheet Fluorescence Microscopy and Two-photon confocal imaging) for unveiling cellular features.

Dr. Accardo is author of 38 publications in peer-reviewed journals (of which 23 as first/corresponding author, h-index=18 as of October 2020), 3 book chapters (of which 1 as first author) and principal inventor of 1 industrial patent on electrowetting superhydrophobic surfaces. His work has been featured in Nature Photonics, Materials Today, PNAS, Small, ACS Applied Materials & Interfaces and Nanoscale.

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Laboratory Webpage

Angelo Accardo

Research projects

Design, fabrication and characterization of 3D/4D Engineered Cell Microenvironments 

  • Light-assisted 3D manufacturing of neuronal microenvironments (PI, TU Delft start up grant, 1 PhD position, 200 k€, 2019)
  • Development of 3D Onco-Scaffolds as a benchmark tool for Proton Radiobiology (PI, TU Delft Health Initiative Pilot Project, 10 k€, 2020) 
  • 3mE Sectorplan Cohesion grant for the project “Bio-mimetic meta-implants” (PI, in collaboration with Mohammad Mirzaali, 200 k€, 2020)
  • DAI (Delft Artificial Intelligence) Lab grant for the project “Forward design of 4D shape-morphing biocompatible structures” (PI, 265 k€ for 1 PhD position) part of the new Artificial Intelligence MACHINA lab that I am co-leading (total of 4 PhD positions).
Teaching

​Academic year 2018/2019

  • “Manufacturing for the micro and nano-scale” (ME46025, MSc Course-Q4), guest lecturer

​Academic year 2019/2020

  • "Intro to Nanoscience and Technology" (ME46010, MSc Course-Q3), guest lecturer.
  • “Manufacturing for the micro and nano-scale” (ME46025, MSc Course-Q4), guest lecturer.

Academic year 2020/2021

  • “Micro and Nanofabrication for Cell Biology and Tissue Engineering” (ME46125, MSc Course-Q4), responsible instructor.
Open and forthcoming positions

MSc assignments are available on a regular basis. The list below provides an example of typical projects. If you are interested to work on projects related to the micro-fabrication and characterization of 3D architectures for cell culture applications, do not hesitate to contact me.

  • 3D fabrication and mechanical characterization of polymer/hydrogel 3D scaffolds for cell culture
  • Advanced photonic 3D fabrication and 3D imaging techniques for guiding and unveiling the differentiation of neural stem cells
  • Mechanobiology aspects of neural cells within 3D free-standing architectures (forthcoming)
  • Combination of conductive polymers and hydrogel materials for smart 3D neuronal microenvironments (forthcoming)
  • Meso-scale porous hydrogel architectures for the culture of neuronal tissue-like models (i.e. neurospheroids, forthcoming)
Key publications
  • D. Fan, U. Staufer, A. Accardo*, Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications, Bioengineering, 2019, 6 (4), 113.
  • A. Accardo, C. Cirillo, S. Lionnet, C. Vieu and I. Loubinoux, Interfacing cells with microengineered scaffolds for neural tissue reconstruction, Brain Research Bulletin, 2019, 152, 202-211.
  • A. Accardo*, R. Courson, R. Riesco, V. Raimbault and L. Malaquin, Direct laser fabrication of meso-scale 2D and 3D architectures with micrometric feature resolution, Additive Manufacturing, 2018, 22, 440-446.
  • A. Accardo*, M.-C. Blatché, R. Courson, I. Loubinoux, C. Vieu and Laurent Malaquin, Direct laser fabrication of free-standing PEGDA-hydrogel scaffolds for neuronal cell growth, Materials Today, 2018, 21 (3), 3156-316.
  • A. Accardo*, M.-C. Blatché, R. Courson, I. Loubinoux, C. Vieu and L. Malaquin, Two-photon lithography and microscopy of 3D hydrogel scaffolds for neuronal cell growth, Biomed. Phys. Eng. Express, 2018, 4, 027009.
  • A. Accardo*, M.-C. Blatché, R. Courson, I. Loubinoux, C. Thibault, L. Malaquin and Christophe Vieu, Multi-photon Direct Laser Writing and 3D Imaging of Polymeric Freestanding Architectures for Cell Colonization, Small, 2017, 13 (27), 1700621.