Thesis defence D.V. Verschueren: single molecule sensing
24 September 2018 15:00 - Location: Aula, TU Delft - By: webredactie
Plasmonic Nanopores for Single Molecule Sensing. Promotor: Prof.dr. C. Dekker (TNW).
Grabbing a single biomolecule and inspecting its contents, without squashing, adapting, or destroying it is far from easy. The work in this thesis is exactly aimed to develop a practical solution to that problem by creating a plasmonic nanopore sensor that can investigate and manipulate single biomolecules. The plasmonic nanopore sensor constructed from two single-molecule sensing devices merged into one: a solid-state nanopore, a tiny hole in a thin membrane that confines a static electric field, and a plasmonic nanoantenna, a gold nanostructure that concentrates light into nanoscale volumes (hotspots). Using these localized static and optical fields, biomolecules can be captured, trapped, perturbed, manipulated, and probed in a variety of ways. This thesis describes a thorough study of this newly designed biosensor. We report on new fabrication techniques to create nanopores and plasmonic nanopores at high throughput and lower cost than existing techniques. We demonstrated that the plasmonic nanopore works as a sensor that can actively pull DNA and protein molecules towards it, can be used to heat up the sensor locally, provides an additional all-optical readout, without the need for any chemical labeling. Furthermore, we demonstrate the sensor can optically trap large protein molecules by virtue of the antenna hotspots to provide ample read-out time. The merging of the two sensors types into one patches the drawback each of them bares separately, creating a novel and more powerful single molecule biosensor that allows control of the single molecule by the experimentor, one single molecule at a time.
For access to theses by the PhD students you can have a look in TU Delft Repository, the digital storage of publications of TU Delft. Theses will be available within a few weeks after the actual thesis defence.