Dr. Stan Brouns
Research Themes: Microbiology
Research Interests: CRISPR-Cas, Bacteriophage resistance, Bacteriophage therapy
Biography
My research group at Delft University of Technology is built around the theme of microbe – bacteriophage interactions, and tackles important questions related to how bacteria become resistant to phage infection and how bacteriophages escape defense systems. This theme is highly relevant for potential clinical use of bacteriophages to treat infections by antibiotic resistant infectious bacteria. I have founded a national open source bacteriophage biobank (www.fagenbank.nl) that can serve as a resource for phage therapy research. We combine microbiology, biochemistry and virology with biophysics and bioinformatics.
For further information regarding current research and available student projects, visit https://www.brounslab.org/
For further information regarding current research and available student projects, visit https://www.brounslab.org/
Courses
Current Projects
- CRISPR-Case adaptation
- CRISPR-Case inhibition
- CRISPR-Cas visualization
- Bacteria in microfluidic flow cells
- Bacteriophage defense
- Bacteriophage isolation
- Bacteriophage engineering
- Bioinformatics - comparative genomics
- DNA sequencing
- Antibiotic resistant bacteria
Highlight Publications
1. Small CRISPR RNAs guide antiviral defense in prokaryotes
Stan JJ Brouns, Matthijs M Jore, Magnus Lundgren, Edze R Westra, Rik JH Slijkhuis, Ambrosius PL Snijders, Mark J Dickman, Kira S Makarova, Eugene V Koonin, John Van Der Oost
In Science 321 (5891), 960-964
2008
2. Evolution and classification of the CRISPR–Cas systems
Kira S Makarova, Daniel H Haft, Rodolphe Barrangou, Stan JJ Brouns, Emmanuelle Charpentier, Philippe Horvath, Sylvain Moineau, Francisco JM Mojica, Yuri I Wolf, Alexander F Yakunin, John Van Der Oost, Eugene V Koonin
In Nature Reviews Microbiology 9 (6), 467-477
2011
3. An updated evolutionary classification of CRISPR–Cas systems
Kira S Makarova, Yuri I Wolf, Omer S Alkhnbashi, Fabrizio Costa, Shiraz A Shah, Sita J Saunders, Rodolphe Barrangou, Stan JJ Brouns, Emmanuelle Charpentier, Daniel H Haft, Philippe Horvath, Sylvain Moineau, Francisco JM Mojica, Rebecca M Terns , Michael P Terns, Malcolm F White, Alexander F Yakunin, Roger A Garrett, John Van Der Oost, Rolf Backofen, Eugene V Koonin
In Nature Reviews Microbiology 13 (11), 722-736
2015
4. Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence
Ekaterina Semenova, Matthijs M Jore, Kirill A Datsenko, Anna Semenova, Edze R Westra, Barry Wanner, John Van Der Oost, Stan JJ Brouns, Konstantin Severinov
In Proceedings of the National Academy of Sciences 108 (25), 10098-10103
2011
5. Structural basis for CRISPR RNA-guided DNA recognition by Cascade
Matthijs M Jore, Magnus Lundgren, Esther Van Duijn, Jelle B Bultema, Edze R Westra, Sakharam P Waghmare, Blake Wiedenheft, Ümit Pul, Reinhild Wurm, Rolf Wagner, Marieke R Beijer, Arjan Barendregt, Kaihong Zhou, Ambrosius PL Snijders, Mark J Dickman, Jennifer A Doudna, Egbert J Boekema, Albert JR Heck, John Van Der Oost, Stan JJ Brouns
In Nature structural & molecular biology 18 (5), 529
2011
