Andor Confocal Spinning Disk

Imaging configuration: Confocal spinning disc / widefield

Microscope stand: Olympus IX81, Inverted
Illumination and detection system: Andor Revolution and Yokogawa CSU X1

Fluorescence excitation: CW lasers, Andor Revolution 

Laser Line (nm)

Max output power (mW)

405

100

445

40

491

100

515

50

561

50

640

100

Detection: 1 channel
Detection devices: EM-CCD Andor iXon X3 DU897
Chip size: 512x512 pixels
Pixel size: 16x16 µm
Dynamic range: 14 bit
Maximum frame rate: 35 frames/s (full chip)
Quantum efficiency: > 90% @ 600 nm

Control and acquisition software: Andor IQ3

Additional features:
FRAPPA unit for FRAP experiment
XY motorized stage
Z piezo stage (+- 100 µm)
Z drift compensation module Olympus ZDC2
Incubator box from OKO Lab for temperature control

Objectives (Olympus) 

Type

Magnification

NumericalAperture (NA)

Medium

Contrast

Working distance (nm)

PlanN

20x

0.4

Air

Fluo

1.2

UPlanFLN

60x

1.25 - 0.65

Oil

Fluo, DIC

0.12

UPlanSApo

100x

1.4

Oil

Fluo, DIC

0.13

Dichroic mirror for Spinning disc illumination

Laser Lines transmitted

Reference

405/488/561/640

Di01-T405/488/568/647

others to be determined

 Fluorescence emission filters 

Emission filter

Type

Reference

542/27

Band-pass

FF01-542/27

617/73

Band-pass

FF01-617/73

685/40

Band-pass

FF01-685/40

690LP

Long-pass 

HQ690LP

447/55 

Band-pass 

FF01-447/55

475/42

Band-pass 

FF01-475/42

525/50 

Band-pass

FF01-525/50

Filter cubes for installed in the microscope for conventional epi-fluorescence

Fluorophore

Excitation filter 

Dichroic filter 

Emission filter 

Filter cube reference

DAPI

387/11 

409 

447/60 

DAPI-1160B-OMF-ZERO

CFP

438/24 

458

483/32 

CFP-2432B-OMF-ZERO

GFP

482/18 

495

520/28 

GFP-1828A-OMF-ZERO

YFP

500/24 

520

542/27 

YFP-2427B-OMF-ZERO

Cy5

628/40 

660

692/40 

Cy5-4040B-OMF-ZERO

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