Nikon confocal A1R/Sim

Imaging configuration: laser scanning / confocal

structured illumination / widefield (SIM)

Microscope stand: Nikon Eclipse Ti, inverted

Laser scanning confocal microscope:

Fluorescence excitation: CW lasers

Laser line (nm)

Max output power (mW)

Ref

405

100

Coherent Cube

457

Melles-Griot IMA 1010 40 ALS

477

Melles-Griot IMA 1010 40 ALS

488

16

Melles-Griot IMA 1010 40 ALS

514

13

Melles-Griot IMA 1010 40 ALS

561

20

Coherent Sapphire

640

40

Coherent Cube

Detection: 4 channels

Detection devices: 2x conventional PMTs (10-20% quantum efficiency)

2x GaAsP (40% quantum efficiency between 450-600 nm)

Control and acquisition software: Nikon NIS

Additional features:
XY mechanical stage
Z piezo stage (+- 100 µm)
Z drift compensation module Nikon Perfect Focus System (PFS)
Galvano scanner mirrors
Resonant scanner mirrors
Transmitted image acquisition
Incubator chamber from Tokai Hit for temperature and Air/CO2 mixing control

Objectives (from Nikon)

Type

Magnification

Numerical Aperture (NA)

Medium

Contrast

Working Distance (mm)

Plan Apo λ

10x

0.45

Air

Fluo, DIC

4

Plan Apo λ

20x

0.75

Air

Fluo, DIC

1

Plan Apo IR

60x

1.27

water

Fluo, DIC, Phase

0.17

SR Apo TIRF

100x

1.49

oil

Fluo, DIC, Phase

0.12

Excitation dichroics
- 405/488
- 405/488/561
- 405/488/561/640
- 400-457/514
- 405/488/543/640/
- Beam splitter 20/80

Channel 1: conventional PMT detector

Dichroic beam-splitter

fluorescence emission filter

495 LP

450/50

515LP

482/35

Channel 2: GaAsP detector

Dichroic beam-splitter

fluorescence emission filter

560 LP

525/50

640LP

540/30

Channel 3: GaAsP detector

Dichroic beam-splitter

fluorescence emission filter

640LP

595/50

Channel 4: conventional PMT detector

Dichroic beam-splitter

fluorescence emission filter

 

700/75

Structured illumination microscope (SIM):

Fluorescence excitation: CW lasers

Laser line (nm)

Max output power (mW)

Ref

457

 

Coherent

488

 

Coherent

515

 

Coherent

561

 

Coherent

640

 

 

Detection: 1 channel
Detection devices: EM-CCD Andor iXon X3 DU897
Chip size: 512x512 pixels
Pixel size: 16x16 µm
Dynamic range: 14 bit
Maximum frame rate: 35 frames/s (full chip)
Quantum efficiency: > 90% @ 600 nm

Control and acquisition software: Nikon NIS

Additional features:
XY mechanical stage
Z piezo stage (+- 100 µm)
Z drift compensation module Nikon Perfect Focus System (PFS)
2D SIM / 3D SIM / widefield
TIRF SIM
Incubator chamber from Tokai Hit for temperature and Air/CO2 mixing control

Objectives (from Nikon)

Type

Magnification

Numerical Aperture (NA)

Medium

Contrast

Working Distance (mm)

SR Apo TIRF

100x

1.49

Oil

Fluo, DIC

0.12

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