Olympus TIRF

Imaging configuration: Widefield

Microscope stand: Olympus IX81, Inverted
TIRF illumination system: Olympus
Dual channel detection system: Andor

Fluorescence excitation: Olympus CW lasers

LASER LINE (NM)

MAX OUTPUT POWER (mW)

405

100

488

150

561

150

640

100

Detection: 2 channels
Detection devices: 2x EM-CCD, Andor iXon X3 DU897
Chip size: 512x512 pixels
Pixel size: 16x16 µm
Dynamic range: 14 bit
Maximum frame rate: 35 frames/s (full chip)
Quantum efficiency: > 90% @ 600 nm

Control and acquisition software: Olympus cell^TIRF and Andor IQ3

Additional features:
XY motorized stage
XYZ piezo stage (+- 100 µm)
Z drift compensation module Olympus ZDC2
Incubator box from OKO Lab for temperature control

Objectives (Olympus)

TYPE

MAGNIFICATION

NUMERICAL APERTURE

MEDIUM

CONTRAST

WORKING DISTANCE (MM)

ApoNTIRF 

60x

1.49

Oil

Fluo, DIC

0.1

UPlanFLN

100x

1.3

Oil

Fluo, DIC, phase

0.2

UApoNTIRF

150x

1.45

Oil

Fluo, DIC

0.08

Dichroic mirrors for TIRF illumination installed in the microscope 

LASER LINES REFLECTED

REFERENCE

405/488/561/640

Di01-R405/488/561/635

488/561

Di01-R488/561

Detection splitting filter cubes for dual channel detection 

Dichroic filter 

Emission filter ch1 

Emission filter ch2 

Filter reference

552

524/29 

630/92 

FF552-Di02, FF01-630/92, FF01-630/92

580

537/26 

630/69 

FF580-FDi01, FF01-537/26, FF01-630/69

662

617/73 

OPEN

FF662-FDi01, FF01-617/73

 Filter cubes installed in the microscope for conventional epi-fluorescence

Fluorophore

Excitation filter 

Dichroic filter 

Emission filter 

Filter cube reference

GFP

482/18 

495

520/28 

GFP-1828A-OMF-ZERO

mCherry

562/40 

593

641/75 

mCherry-B-OMF-ZERO

Cy5

628/40 

660

692/40 

Cy5-4040B-OMF-ZERO

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