Olympus Widefield

Imaging configuration: Widefield

Microscope stand: Olympus IX81, inverted

Fluorescence excitation: High-power lamp Andor AMH-200-F6S

Detection: 1 channel
Detection device: EM-CCD Andor Luca R
Chip size: 1004x1002 pixels
Pixel size: 8x8 ┬Ám
Dynamic range: 14 bit
Maximum frame rate: 12 frames/s (full chip)
Quantum efficiency: 65% @ 600 nm

Control and acquisition software: Andor IQ3

Additional features:
XY motorized stage
Z drift compensation module Olympus ZDC2
Incubator box from OKO Lab for temperature and Air/CO2 mixing control

Objectives (from Olympus) 

TYPE

MAGNIFICATION

NUMERICAL APERTURE (NA)

MEDIUM

CONTRAST

WORKING DISTANCE (MM)

UPlanFLN  

10x

0.3

Air

Fluo, DIC

10

UPlanFLN

20x

0.5

Air

Fluo, DIC, phase

2.1

PlanN

40x

0.65

Air

Fluo

0.6

PlanApoN

60x

1.42

Oil

Fluo, DIC

0.15

UplanSApo

100x

1.4

Oil

Fluo

0.13

UPlanFLN

100x

1.3

Oil

Fluo, DIC, Phase

0.2

 Filter Cubes installed in the microscope

Fluorophore

Excitation filter

Dichroic filter

Emission filter 

Filter cube reference

DAPI

387/11 

409

447/60 

DAPI-1160B-OMF-ZERO

GFP

482/18 

495

520/28 

GFP-1828A-OMF-ZERO

YFP

500/24

520

542/27 

YFP-2427B-OMF-ZERO

mCherry

562/40 

593

593LP

mCherry-40LP-A-OMF-ZERO

Cy5

628/40

660

692/40

Cy5-4040B-OMF-ZERO

/* */