Picoquant MicroTime 200

Imaging configuration: Confocal for time-resolved fluorescence measurements (FCS, FLIM, FRET)

Widefield

Microscope stand: Olympus IX81, Inverted
Illumination and detection system: Picoquant MicroTime 200

Confocal detection:

Fluorescence excitation: Picoquant picosecond pulsed lasers

Laser line (nm)

Max output power @ 40 MHz (mW)

Pulse duration (ps)

404

3

54

485

5

93 (2 laser sources with orthogonal polarization)

530

2

83

640

4

63

Detection: 2 channels
Detection devices: 2x SPAD from Micro Photon Devices (MPD), PDM series
Single-photon detectors
Quantum efficiency: 50% @ 550 nm
Timing accuracy: < 50ps

Control and acquisition software: Picoquant SymPhoTime

Additional features:
XY mechanical stage
Objective mounted on XYZ piezo stage (2D + PIFOC, +- 100 µm)
Z drift compensation module Olympus ZDC2
Fully equipped for conventional widefield epi-fluorescence imaging (see below for details)

Objectives (from Olympus)

Type

Magnification

Numerical Aperture (NA)

Medium

Contrast

Working distance (mm)

UPlanSApo

60x

1.2

water

fluo, DIC

0.28

Dichroic mirror for confocal illumination

Laser lines reflected

reference

530/640

Z532/635 rpc

480/640

480/640 FITC/Cy5

405

ZT405 rdc

480/530

Z480/532 rpc

Fluorescence emission filters

Emission filter

Type

Reference

520/35

Band-pass

FF01-520/35 (x2)

542/25

Band-pass

FF01-542/25

690/70

Band-pass 

HQ690/70

430LP

Long-pass

HQ430LP

563/9

Band-pass

HC563/9

580/70

Band-pass

HQ580/70

Dichroic beam-splitter for dual-channel detection

Dichroic filter

reference

620

620DCXXR (x2)

500

500DCXR

532

Z532 RDC

638

Z638 RDC

Confocal pinholes available: 30, 50, 75, 100, 150 µm

Widefield detection:

Fluorescence excitation: High-power lamp Andor AMH-200-F6S

Detection: 1 channel
Detection device: EM-CCD Andor Luca R
Chip size: 1004x1002 pixels
Pixel size: 8x8 µm
Dynamic range: 14 bit
Maximum frame rate: 12 frames/s (full chip)
Quantum efficiency: 65% @ 600 nm

Control and acquisition software: Andor IQ3

Widefield epi-fluorescence filter cubes installed in the microscope

Fluorophore

Excitation filter

Dichroic filter

Emission filter

Filter cube reference

GFP

482/18

495

520/28

GFP-1828A-OMF-ZERO

TRITC

443/22

562

593/40

TRITC-B-OMF-ZERO

Cy5

628/40

660

692/40

Cy5-4040B-OMF-ZERO

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