Nuclear Shuttling

Nuclear shuttling dynamics of Huntingtin Exon 1 mutants

What? Bachelor/Master project to investigate defective nucleo-cytoplasmic transport, i.e. the trafficking
of macromolecules between the nucleus and cytoplasm, in Huntington’s Disease. Huntington’s disease is
an incurable neurodegenerative disease caused by an expanded CAG repeat in the Huntingtin (Htt) gene.
You will set up super-resolution imaging of nuclear pore complex and single particle tracking of mutant
Huntingtin protein variants

Starting date & contact: summer 2023 onwards; Moritz Engelhardt, Email: m.l.k.engelhardt@tudelft.nl
Department/Faculty: BN/AS

For you? You learn: Cell culture and labeling, state-of-the-art super-resolution microscopy methods ,
advanced image analysis as well as visualization and presentation of experimental data. Depending on
your background, the project may include additional data simulation related to the NPC shuttling. Your
background: (bio-) physics/engineering, nanobiology, biochemistry or related.

Extended project description