Nuclear Shuttling

Nuclear shuttling dynamics of Huntingtin Exon 1 mutants

What? Bachelor/Master project to investigate defective nucleo-cytoplasmic transport, i.e. the trafficking
of macromolecules between the nucleus and cytoplasm, in Huntingtonā€™s Disease. Huntingtonā€™s disease is
an incurable neurodegenerative disease caused by an expanded CAG repeat in the Huntingtin (Htt) gene.
You will set up super-resolution imaging of nuclear pore complex and single particle tracking of mutant
Huntingtin protein variants

Starting date & contact: summer 2023 onwards; Moritz Engelhardt, Email: m.l.k.engelhardt@tudelft.nl
Department/Faculty: BN/AS

For you? You learn: Cell culture and labeling, state-of-the-art super-resolution microscopy methods ,
advanced image analysis as well as visualization and presentation of experimental data. Depending on
your background, the project may include additional data simulation related to the NPC shuttling. Your
background: (bio-) physics/engineering, nanobiology, biochemistry or related.

Extended project description

Huntingtin Exon 1 library nuclear shuttling dynamics visu- alized with multiplane imaging system. Different posttranslational modifications (PTMs) of HttEx1 are shown to influence nuclear lo- calisation of aggregates, influenced by the NPC gating function. Experimental figures are taken from Chowdhury et al., Nat Cell Biol 24, 400 (2022).