Martin Pabst Group
The focus of the Protein Mass Spectrometry Group is on exploring the post-translational protein modification repertoire of microbial cell systems by using high-resolution and quantitative mass spectrometry.
The complexity in a cellular system is mostly at the level of protein variation, rather than due to the number of distinct genes. The vastly higher diversity of the proteome compared to the genome is by a significant proportion introduced through post-translational modifications. These modifications provide an elegant solution to modulate protein properties at a physiological time scale, such as fast regulation of intermolecular interactions or tuning of enzyme activities. Our focus here is on exploring microbial cell systems with relevance for industrial applications.
We are also aiming to gain further insight into how microbes work together, by characterising the composition of microbial communities using meta-proteomics and by analysing exopolymeric substances such as proteins or carbohydrates and their interaction with other components of the matrix.
Our laboratory is equipped with micro- and nano-flow chromatographic systems coupled to state-of-the-art mass spectrometers (Orbitrap-MS, Q-TOF-MS, TQ-MS), bioinformatics tools for data processing, HPLC-FLD and CE systems.