Pascale Daran-Lapujade Group

Research

Fascinated by the complexity of biological systems, my research aims at bringing understanding on how microbes respond to their environment and at using this knowledge to turn cells in powerful factories. My research focuses on the fantastic model eukaryote and industrial yeast Saccharomyces cerevisiae, with a growing interest for ‘non-conventional’ yeasts. My research is articulated around two major themes, the study of central carbon metabolism and its regulation, and uncoupling growth from product formation. My projects integrate a broad range of approaches (e.g. quantitative physiology, genomics, cell biology) and of techniques either from our own section (e.g. molecular biology toolbox, cultivation in bioreactors, transcriptome analysis, fluorescence microscopy, FACS, etc.)  or in collaboration (e.g. proteomic, metabolomics). Realizing that the limited genetic accessibility of S. cerevisiae was hampering the study of its central carbon metabolism, I ventured in the field of synthetic biology a few years ago. I developed a new research line aiming at constructing yeast platform that enable to rapidly and easily remodel essential pathways. This ambitious research line has recently gained momentum thanks to the support of an ERC Consolidator grant.

Teaching

I am very active in the Life Science & Technology curricula from our department. At the bachelor level I coordinate a large practicum teaching the basics of microbiology, fermentation and spectroscopy (course LB1062, 6 EC). This practicum is taught by a team of enthusiastic technicians and PhD students and gives a solid basis to the 1st year students. I challenge the LST master students with the critical analysis of scientific literature in the field of metabolic engineering (course LM3442, 6 EC), in a very interactive and stimulating course. Finally as chair of the Board of Examiners I contribute to the quality of our LST master program.

I also contribute to various advanced courses organized by Biotech Delft. 

BSc programme Life Science and Technology

Delft University of Technology and Leiden University

BSc Life Science & Technology 

Biotechnology Basic Techniques, BBT1

Course code: LB1062
Coordinator Pascale Daran-Lapujade; 6 EC

Acquiring practical skills, ranging from the handling of microorganisms, through pipetting to working with bioreactors, is essential for both Life Science and Technology and the research in our group. Not surprisingly, many PhD students and staff technicians of the IMB group supervise the 1st or 2nd year Biotechnology practicals.

Reading materials for this practical are in Dutch. Supervision English/Dutch.

1st and 2nd year Biotechnology practicals

IMB Staff Technicians and PhD students

Acquiring practical skills, ranging from the handling of microorganisms, through pipetting to working with bioreactors, is essential for both Life Science and Technology and the research in our group. Not surprisingly, many PhD students and staff technicians of the IMB group supervise the 1st or 2nd year Biotechnology practicals.

Reading materials for this practical are in Dutch. Supervision English/Dutch.

MSc programme Life Science and Technology

Delft University of Technology

MSc Life Science & Technology

Metabolic Reprogramming

Course code: LM3442
Pascale Daran-Lapujade, 6EC

Metabolic Reprogramming focuses on strategies to optimize industrial micro-organisms and equip them with new functionalities. Lectures are combined with intensive discussions on selected scientific publications. Each student prepares a presentation on one scientific publication; all students read the complete set of publications and actively engage in group discussions on the papers. In addition to scientific content, attention is given to other aspects of the scientific publications (style, statistics, internal consistency, impact).

This course is taught in English

Other activities

To animate the Dutch Biotech community I chair the joint Microbial Biotechnology section of the KNVM and Microbial Physiology workgroup of the NBV. With a group of motivated board members we organise a one-day fall meeting on the theme of Microbial Biotechnology, taking place on a yearly basis on the first Monday of November.

Since 2014 I am senior editor at the international journal FEMS Yeast Research.

Dr P.A.S. Daran-Lapujade

Associate professor