Olympus Widefield
Imaging configuration: Widefield
Microscope stand: Olympus IX81, inverted
Fluorescence excitation: High-power lamp Andor AMH-200-F6S
Detection: 1 channel
Detection device: EM-CCD Andor Luca R
Chip size: 1004x1002 pixels
Pixel size: 8x8 µm
Dynamic range: 14 bit
Maximum frame rate: 12 frames/s (full chip)
Quantum efficiency: 65% @ 600 nm
Control and acquisition software: Andor IQ3
Additional features:
XY motorized stage
Z drift compensation module Olympus ZDC2
Incubator box from OKO Lab for temperature and Air/CO2 mixing control
Objectives (from Olympus)
TYPE | MAGNIFICATION | NUMERICAL APERTURE (NA) | MEDIUM | CONTRAST | WORKING DISTANCE (MM) |
UPlanFLN | 10x | 0.3 | Air | Fluo, DIC | 10 |
UPlanFLN | 20x | 0.5 | Air | Fluo, DIC, phase | 2.1 |
PlanN | 40x | 0.65 | Air | Fluo | 0.6 |
PlanApoN | 60x | 1.42 | Oil | Fluo, DIC | 0.15 |
UplanSApo | 100x | 1.4 | Oil | Fluo | 0.13 |
UPlanFLN | 100x | 1.3 | Oil | Fluo, DIC, Phase | 0.2 |
Filter Cubes installed in the microscope
Fluorophore | Excitation filter | Dichroic filter | Emission filter | Filter cube reference |
DAPI | 387/11 | 409 | 447/60 | DAPI-1160B-OMF-ZERO |
GFP | 482/18 | 495 | 520/28 | GFP-1828A-OMF-ZERO |
YFP | 500/24 | 520 | 542/27 | YFP-2427B-OMF-ZERO |
mCherry | 562/40 | 593 | 593LP | mCherry-40LP-A-OMF-ZERO |
Cy5 | 628/40 | 660 | 692/40 | Cy5-4040B-OMF-ZERO |
