Mechanobiology of in vitro cartilage tissues (BEP/MEP)

Short description:
In connective tissues, cells are embedded in a support matrix composed of a complex 3D network of extracellular macromolecules and proteins. Cells receive various biophysical stimuli through this extracellular matrix (ECM) such as topographic cues or mechanical stimulation.

For instance, chondrocytes, specialized collagen-secreting cells responsible for the maintenance of cartilaginous tissues within joints, are constantly exposed to mechanical loading (compression, shear). This project aims at understanding the interplay between cell behaviour and ECM properties using in vitro model systems based on 3D cultures of chondrocytes in cartilage-mimicking hydrogels and/or the influence of controlled external loading on the cell response. The goal is to gain insights into chondrocyte mechanobiology in healthy but also in diseased conditions.

Techniques/methods applied in the project:
In this project, you will gain expertise in microscopy, cell culture and mechanical testing in the context of biological tissues.

Relevant publication for the project:
The role of cell–matrix interactions in connective tissue Mechanics, I. Muntz, M. Fenu , G.J.V.M. van Osch, G.H. Koenderink, Physical Biology, 2022

Contact
Irene Nagle (I.Nagle@tudelft.nl) and Prof. Dr. Gijsje Koenderink (G.H.Koenderink@tudelft.nl)