Cytoskeletal crosstalk in confined cancer cells (MEP)
Short description:
Confinement parameters of extracellular matrix in the local tumor microenvironments can impact tumor cell migration strategies and therefore also invasion efficiency and metastatic potential. To better understand the effect of confinement on cancer cell morphology and mechanics, we use live-cell confocal microscopy to study the localization of different cytoskeletal networks and their crosstalk in genetically manipulated cancer cells that are confined on 2D micropatterns that mimic 3D collagen migration tracks. Via image analysis, we aim to elucidate strategies employed by cancer cells to survive extreme confining conditions via the cytoskeletal networks.
Techniques/methods applied in the project:
- Micropatterning
- Live-cell confocal microscopy
- Cell culture
- Electroporation
- Image analysis
Contact
Anouk van der Net (J.J.P.vanderNet@tudelft.nl) and Prof. Dr. Gijsje Koenderink (G.H.Koenderink@tudelft.nl)