Olympus widefield for long term imaging
Purpose:
- Suitable for fluorescence, transmitted and phase imaging
- Suitable for live samples
- When no optical sectioning capability is required
- Large field-of-view observations
- Option for Microfluidics
Illumination / Imaging configuration: Widefield
Imaging contrasts: Brightfield, Phase contrast and Fluorescence
Microscope stand: Inverted Olympus IX81, motorized
Fluorescence excitation source WF: CoolLED pE-4000, LED light source
Detection: 1 channel
Detection device: Andor life 888
| Camera | Andor Life 888 |
| Technology | EM-CCD |
| Chip size | 1024x1024 pixels |
| Pixel size | 13x13 µm |
| Dynamic range | 16-bit |
| Max frame rate* | 26 frames s-1 |
| Quantum efficiency | 95% @ 550 nm |
*full chip, no binning
Acquisition software: µManager 2.0-gamma
Additional features:
- XY motorized stage
- Hardware Z-drift compensation (Olympus ZDC2)
- Incubation box for temperature control, Air/CO2 mixing in option
Objectives:
| Objective type | Mag/NA | Medium | Contrast | WB (mm) | Z-Drift compensation system | Pixel size (nm) |
| UPlanFLN | 10x / 0.3 | Air | Fluo, Ph | 10 | / | 795 |
| UPlanSApo | 40x / 0.95 | Air | Fluo | 0.18 | / | 325 |
| APON | 60x / 1.4 | Oil | Fluo | 0.12 | YES | 216 |
| UplanSApo | 100x / 1.45 | Oil | Fluo, Ph3 | 0.13 | YES | 130 |
Fluorescence filter cubes installed:
Fluorophore | Excitation filter | Dichroic filter cut-off | Emission filter | Filter cube reference |
DAPI | 387/11 | 409 | 447/60 | DAPI-1160B |
GFP | 482/18 | 495 | 520/28 | GFP-1828A |
YFP | 500/24 | 520 | 542/27 | YFP-2427B |
mCherry | 562/40 | 593 | 593LP | mCherry-40LP-A |
