Spinning Disc Confocal

Purpose:
- Fast and gentle 3D confocal sectioning @ Large Field of View 
- Live experiments 
- Smart acquisition using Nikon NIS.ai 
- FRAP

Microscope stand: Nikon Eclipse Ti-2, inverted, equipped with environmental control
Spinning disc unit: Crest X-light V3. 50um pinhole size with 250um pinhole spacing
Fluorescence excitation source: CELESTA light engine, 405/446*/477/520*/546/638/749
*requires additional dichroic to be installed- not currently available
solid state class 4 lasers@120mW: 405nm; 488nm; 561nm(200mW); 638nm
Integrally installed bandpass filters per line, incl.multi band cube for fast acquisition
Fluorescence Emission filters: DA/FI/TR/Cy5/Cy7

Detection: 1 channel
Detection device: Photometrics Kinetix sCMOS
Active array size: 3200 x 3200 (10.24 Megapixel)
Sensor Area: 29.4mm diagonal
Pixel size: 6.5x6.5 µm
Dynamic range: 16 bit
Maximum frame rate: 83 frames/s (full chip)
Quantum efficiency: > 95%

Control and acquisition software: Nikon NIS
Additional features:
- XY mechanical stage
- Z piezo stage (+- 200 µm)
- Z drift compensation module Nikon Perfect Focus System (PFS)
- Transmitted image acquisition
- Incubator chamber from OkoLab control of temperature, humidity and CO₂ levels

Objectives (from Nikon):